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Galactose terminated oligosaccharides activate macrophage respiratory burst.

Aparna, H. S. and Salimath, P. V. (1994) Galactose terminated oligosaccharides activate macrophage respiratory burst. Nutrition Research, 14 (3). 433-444; 31 ref..

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The results of activation of the respiratory burst by lactose and sialyl lactose which were comparable to those of known activators such as phorbol ester, lipopolysaccharide and Ca2+-ionophore, are presented. These oligosaccharides were used as models for erythrocyte membrane glycoproteins whose terminal sugars are galactose or sialic acid. Maximum activation at sialyl lactose or lactose concentrations as low at 3 or 6 pM was obtained while desialylated erythrocyte membrane (5 ng) with exposed beta-galactosyl residues containing small amounts of sialic acid proved to be effective activators of respiratory burst. From the kinetics of activation, it was established that maximum respiratory burst occurred between 5-9 min with oligosaccharides or membranes as activators. The probable mechanism of activation by oligosaccharides and membranes was found to operate via protein kinase C or calmodulin signal mediation pathways and inhibition by intracellular cAMP suggested the role for dephosphorylation and inactivation of respiratory burst. The results suggested that exposed beta-galactosyl residues were capable of triggering the generation of reactive O2 species which are implicated in killing and digestion of the engulfed organisms by phagocytes.

Item Type: Article
Uncontrolled Keywords: erythrocytes-; macrophages-; immune-response; oligosaccharides-; in-vitro
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 31 Sugar Chemistry
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 30 Apr 2012 04:08
Last Modified: 30 Apr 2012 04:08
URI: http://ir.cftri.com/id/eprint/8510

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