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Amyloglucosidase-catalysed synthesis of curcumin-bis-alpha-D-glucoside - a response surface methodological study.

Vijayakumar, G. R. and Balaraman, Manohar and Soundar, Divakar (2006) Amyloglucosidase-catalysed synthesis of curcumin-bis-alpha-D-glucoside - a response surface methodological study. European Food Research and Technology, 223 (6). 725-730 ; 30 ref..

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Official URL: http://dx.doi.org/10.1007/s00217-006-0259-3

Abstract

Response surface methodology (RSM) was employed to optimize amyloglucosidase (glucan 1,4-alpha-glucosidase)-catalysed synthesis of curcumin-bis-alpha-D-glucoside. A central composite rotatable design (CCRD) was employed involving 5 variables (enzyme concn., curcumin concn., incubation period, buffer concn. and pH) at 5 levels. A 2nd-order polynomial equation with an R2 value of 0.9 showed good correspondence between experimental and predicted yields. 3D surface and contour plots described the catalytic efficiency of amyloglucosidase under the reaction conditions employed. A max. conversion rate of 35% was predicted for curcumin concn. >0.55 mmol at 35-60% (w/w D-glucose) amyloglucosidase concn. While lower (<35%) amyloglucosidase concn. converted less, concn. >60% could be inhibitory to curcumin. The extent of glucosylation was governed by a critical buffer (0.95-1.0 ml of 0.01M, pH 6.0) to enzyme ratio (15-45% w/w D-glucose). Experiments under optimum predicted conditions of 16.9% (w/w D-glucose) amyloglucosidase, 0.33 mmol curcumin, 120 h incubation period, 0.1mM (1.0 ml of 0.01M) buffer concn. at pH 7.5 gave a conversion yield of 56.3%. Validation experiments carried out under selected random conditions also showed good correspondence between experimental and predicted yields.

Item Type: Article
Uncontrolled Keywords: AMYLASES-; GLYCOSIDES-; PHENOLS-; PIGMENTS-; CURCUMIN-; GLUCAN-1,4-alpha-GLUCOSIDASES; GLUCOSIDES-
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 10 Spectroscopic and Spectrometric analysis
Divisions: Fermentation Technology and Bioengineering
Depositing User: Users 16 not found.
Date Deposited: 04 Jul 2011 07:05
Last Modified: 28 Aug 2018 05:41
URI: http://ir.cftri.com/id/eprint/8101

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