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Production of steviosides in ex vitro and in vitro grown Stevia rebaudiana Bertoni.

Rajasekaran, T and Giridhar, P and Ravishankar, G. A. (2007) Production of steviosides in ex vitro and in vitro grown Stevia rebaudiana Bertoni. Journal of the Science of Food and Agriculture, 87 (3). 420-424 ; 25 ref..

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Abstract

Concn. of steviosides were determined in leaves, shoots, roots and flowers of Stevia rebaudiana Bertoni. Stevioside extracts were obtained from both 1-month old greenhouse-grown plants and in vitro cultures in a process based on hydrolysis, esterification, evaporation and dissolution in methanol. HPLC analysis of methanol extracts revealed the presence of all 8 known sweet diterpene glycosides of S. rebaudiana, with steviolbioside, stevioside, rebaudiosides C and A and dulcoside A predominating. Steviosides contents generally declined in the order leaves > shoots > roots > flowers. Highest individual stevioside concn. in leaves from greenhouse plants was recorded for steviolbioside (64.80 g/kg dried plant), while highest concn. for leaves from in vitro cultures was for rebaudioside A (0.99 g/kg). Recovery of steviosides from leaves was 90% using methanol extraction.

Item Type: Article
Uncontrolled Keywords: CULTIVATION-; GLYCOSIDES-; PLANTS-; SWEETENERS-; TERPENOIDS-; STEVIA-REBAUDIANA; STEVIOSIDE-
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
500 Natural Sciences and Mathematics > 10 Plants > 01 Plant Cell
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 12 Aug 2008 08:51
Last Modified: 12 Aug 2008 08:51
URI: http://ir.cftri.com/id/eprint/8071

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