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Aqueous two phase extraction for purification of C-phycocyanin.

Ganapathi, Patil and Raghavarao, K. S. M. S. (2007) Aqueous two phase extraction for purification of C-phycocyanin. Biochemical Engineering Journal, 34 (2). pp. 156-164.

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Abstract

Optimization of the downstream processing of C-phycocyanin from cultures of the microalga Spirulina platensis using an aqueous 2-phase extraction process is reported. Effects on the extraction of pH, phase composition and nature and vol. ratio of the phases were assessed. Results show that use of an extraction system comprising polyethylene glycol (4000) and potassium phosphate at a phase vol. ratio of 0.8 and pH of 6 were suitable for extraction of C-phycocyanin from S. platensis. Under these conditions, max. purity was achieved when 3 extractions were performed. Purification of the pigment from the organic extraction solvent could be achieved using ultrafiltration and freeze drying of the purified C-phycocyanin was used to obtain the pigment in powder form.

Item Type: Article
Uncontrolled Keywords: BACTERIA-; DOWNSTREAM-PROCESSING; EXTRACTION-; FERMENTATION-TECHNOLOGY; GLYCOLS-; PHOSPHATES-; PIGMENTS-; PHYCOCYANIN-; POLYETHYLENE-GLYCOL; SPIRULINA-PLATENSIS
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
600 Technology > 08 Food technology > 05 Processing and Engineering
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 12 Aug 2008 11:38
Last Modified: 10 Oct 2018 10:47
URI: http://ir.cftri.com/id/eprint/8060

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