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Effects of growth hormones and maturity of the fruits on the callus culture of guava (Psidium guajava) fruits using response surface methodology.

Madhavi, D. L. and Chand, N. and Rajalakshmi, D. and Patwardhan, M. V. (1992) Effects of growth hormones and maturity of the fruits on the callus culture of guava (Psidium guajava) fruits using response surface methodology. Journal of the Science of Food and Agriculture, 58 (1). 29-34, 14 ref..

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Abstract

Guava, a tropical fruit, is valued for its aroma and is used in many fruit products. Earlier work from this laboratory showed that the expression of the flavour as a secondary metabolite through tissue culture in vitro is possible. In the present study an attempt was made to maximize callus culture growth through optimization of hormonal combinations of indole acetic acid (IAA), 2,4-dichlorophenoxy acetic acid (2,4-D) and kinetin (Kn). Effect of using fruits of different maturity was also assessed. Study of the complex interactions of growth hormones and identifications of the optimal hormonal levels were carried out using appropriate design of experiments. Simplex optimization techniques were used to obtain the optimum levels of these hormones for highest relative growth in the experimental region. Results indicate that callus culturing with fruits before reaching climacteric with MS medium supplemented with 0.01 mg l-1 of IAA and 2,4-D and 2.87 mg l-1 of Kn results in a high relative growth of 8.97 for the callus.

Item Type: Article
Uncontrolled Keywords: AGE-; CELL-CULTURE; FRUITS-SPECIFIC; GUAVAS-; HORMONES-; CALLUS-CULTURE
Subjects: 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
600 Technology > 08 Food technology > 24 Fruits
Divisions: Fruit and Vegetable Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 27 May 2016 06:47
Last Modified: 27 May 2016 06:47
URI: http://ir.cftri.com/id/eprint/7991

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