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Isolation and immunochemical characterization of the alcohol-extractable proteins (kafirins) of Sorghum bicolor (L.) Moench.

Mazhar, H. and Chandrashekar, A. and Shetty, H. S. (1993) Isolation and immunochemical characterization of the alcohol-extractable proteins (kafirins) of Sorghum bicolor (L.) Moench. Journal of Cereal Science, 17 (1). 83-93, 28 ref..

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Journal of Cereal Science, Volume 17, Issue 1, January 1993, Pages 83-93.pdf - Published Version
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Abstract

A simple protocol was developed for purification of the alpha-, beta- and gamma-kafirins (alcohol-extractable proteins) of sorghum based on extractability differences. The relationship between kafirins extractable in 60% t-butanol and cross-linked kafirins extractable in 60% t-butanol/0.6% 2-mercaptoethanol was examined using immunochemical techniques. The immunological relationship between kafirins and prolamins from other tropical millets and cereals was also investigated. Polyclonal antibodies were raised against the kafirins; immunoblotting showed no cross reactivity between the 3 kafirins. N analysis, SDS-PAGE, immunoblotting and ELISA demonstrated the quantitative differences between the kafirins and the cross linked kafirins. ELISA determined that the increased extractability of beta- and gamma-kafirins was responsible for the increase in total kafirin extracted on addition of a reducing agent to aqueous t-butanol. Homologies were thought to exist amongst the prolamins contained in tropical millets and cereals as all 3 kafirin antibodies reacted with the specific prolamins.

Item Type: Article
Uncontrolled Keywords: ANALYTICAL-TECHNIQUES; CEREAL-PRODUCTS; CEREALS-; IMMUNOLOGICAL-TECHNIQUES; PROTEINS-; PROTEINS-CEREAL; SORGHUM-; IMMUNOCHEMICAL-ANALYSIS; KAFIRINS-
Subjects: 600 Technology > 08 Food technology > 21 Cereals > 06 Sorghum
Divisions: Flour Milling Bakery and Confectionary Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 30 Mar 2016 09:37
Last Modified: 04 Sep 2018 11:29
URI: http://ir.cftri.com/id/eprint/7953

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