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Media optimisation for production of lactic acid by Lactobacillus plantarum NCIM 2084 using response surface methodology.

Krishnan, S. and Suvendu, Bhattacharya and Karanth, N. G. (1998) Media optimisation for production of lactic acid by Lactobacillus plantarum NCIM 2084 using response surface methodology. Food Biotechnology, 12 (1/2). pp. 105-121.

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Abstract

For the production of lactic acid by Lactobacillus plantarum NCIM 2084, response surface methodology (RSM) using central composite rotatable design (CCRD) was adopted for optimizing the media composition with respect to liquefied starch, wheat bran extract, ammonium nitrate and sodium acetate. A set of 28 experiments was performed using cannonical analysis. Significant (p <less than or equal to> 0.25) effects were observed for the first 3 variables. Quadratic and interaction effects of variables were more prominent than linear effects. The optimum concn. obtained were: liquefied starch, 9.99; wheat bran extract, 0.05; ammonium nitrate, 2.48; and sodium acetate, 0.08%. A substrate conversion of 92% and a lactic acid concn. of 72.9 g/l in 24 h were obtained under optimized conditions. Results showed that it is possible to replace the expensive yeast extract by a combination of wheat bran extract and ammonium nitrate. [From En summ.]

Item Type: Article
Uncontrolled Keywords: ANALYTICAL-TECHNIQUES; FERMENTATION-PRODUCTS; LACTIC-ACID; LACTOBACILLUS-; MICROBIOLOGICAL-TECHNIQUES; MEDIA-; RESPONSE-SURFACE-METHODOLOGY
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Fermentation Technology and Bioengineering
Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Feb 2012 09:15
Last Modified: 30 May 2012 10:04
URI: http://ir.cftri.com/id/eprint/7781

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