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Production of astaxanthin in Haematococcus pluvialis cultured in various media.

Usha, Tripathi and Sarada, R. and Ramachandra Rao, S. and Ravishankar, G. A. (1999) Production of astaxanthin in Haematococcus pluvialis cultured in various media. Bioresource Technology, 68 (2). 197-199, 16 ref.. ISSN S0960-8524

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Abstract

Production of astaxanthin by Haematococcus pluvialis in autotrophic (Bold Basal (BBM), Z8 or A9 media), heterotrophic (KM1 medium) and mixotrophic (mixotrophic medium 1 (MM1; BBM + sodium acetate), MM2 (MM1 + L-Asn) and KM2 medium (KM1 without yeast extract)) conditions was examined. Growth of H. pluvialis was faster in heterotrophic (KM1) and mixotrophic (MM2 and KM2) media than in autotrophic media. The highest cell count was observed in KM2 medium. In KM1 medium, total carotenoid content was 181.0 pg/cell (88.67% astaxanthin) after 45 days in liquid medium and 462.7 pg/cell (90.45% astaxanthin) after 32 days on agar medium. Addition of trace elements and B vitamins increased astaxanthin production in all mixotrophic media. Max. astaxanthin concn. (2.2% (w/w) yield) was observed in KM2 medium with added trace elements and B vitamins. It is concluded that addition of sodium acetate, L-Asn, trace elements and B vitamins to autotrophic and mixotrophic media increases biomass and total astaxanthin production by H. pluvialis and should therefore be useful for reducing the cost of large scale astaxanthin production.

Item Type: Article
Uncontrolled Keywords: ALGAE-; CAROTENOIDS-; FERMENTATION-PRODUCTS; MICROBIOLOGICAL-TECHNIQUES; ASTAXANTHIN-; HAEMATOCOCCUS-PLUVIALIS; MEDIA-
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 29 Jul 2008 09:48
Last Modified: 30 Dec 2016 08:52
URI: http://ir.cftri.com/id/eprint/7755

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