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Secretion to the growth medium of an alpha-amylase by Escherichia coli clones carrying a Bacillus laterosporus gene.

Manonmani, H. K. and Kunhi, A. A. M. (1999) Secretion to the growth medium of an alpha-amylase by Escherichia coli clones carrying a Bacillus laterosporus gene. World Journal of Microbiology & Biotechnology, 15 (4). 475-480, 19 ref..

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Abstract

The alpha-amylase gene from Bacillus laterosporus P3 was cloned and expressed in Escherichia coli HB101 and DH5alpha. Up to 92% of the cloned gene product was secreted into the medium by recombinant E. coli. The recombinant crude enzyme showed improved functionality in terms of activity at a wider pH range and at higher temp. compared with the crude enzyme from the donor strain. Improved functionality of the cloned enzyme was due to the absence of a contaminating proteinase which was co-produced in the donor strain. Sub-cloning of the alpha-amylase gene using the promoter-probe vector pKT240 in E. coli DH5alpha indicated the presence of a promoter of B. laterosporus P3 in the cloned fragment.

Item Type: Article
Uncontrolled Keywords: AMYLASES-; BACILLUS-; ESCHERICHIA-; GENE-CLONING; GENETICS-; MICROORGANISMS-; alpha-AMYLASES; BACILLUS-LATEROSPORUS; ESCHERICHIA-COLI; RECOMBINANT-MICROORGANISMS
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 30 Jun 2009 09:45
Last Modified: 14 Dec 2016 12:23
URI: http://ir.cftri.com/id/eprint/7726

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