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Megaplasmid encoding novel sugar utilizing phenotypes, pediocin production and immunity in Pediococcus acidilactici C20.

Halami, P. M. and Ramesh, A. and Chandrashekar, A. (2000) Megaplasmid encoding novel sugar utilizing phenotypes, pediocin production and immunity in Pediococcus acidilactici C20. Food Microbiology, 17 (5). 475-483, 27 ref..

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Abstract

Plasmid-linked traits in a strain of Pediococcus acidilactici C20, previously isolated from fowl intestine and revealed to harbour a single megaplasmid, were characterized. Curing experiments using novobiocin (1 mug/ml) in combination with high temp. (25C) were conducted to ascertain plasmid-linked functions. Results indicated that the genetic determinants encoding capacity to ferment a wide range of sugars, pediocin production and immunity against pediocin were associated with the megaplasmid. Strain C20 was able to ferment lactose (not previously reported for pediocin-producing P. acidilactici strains), maltose, mannitol, melibiose, raffinose, sorbitol, sucrose and trehalose. Pediocin activities were observed to range from 2500 to 3500 AU/ml when strain C20 was grown in the presence of different C sources. Rapid molecular techniques such as PCR, dot-blot and other biochemical tests revealed that the pediocin produced by strain C20 was similar to pediocin AcH/PA-1 and had high activity against Listeria monocytogenes. It is concluded that the strong antilisterial activity exhibited by strain C20 in combination with its rare ability to ferment lactose make it a likely candidate for the biocontrol of Listeria in dairy, vegetable and meat products.

Item Type: Article
Uncontrolled Keywords: BACTERIOCINS-; FERMENTATION-; IMMUNOLOGY-; PEDIOCOCCUS-; PLASMIDS-; PEDIOCINS-; PEDIOCOCCUS-ACIDILACTICI
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 17 Jun 2011 04:50
Last Modified: 28 Dec 2011 10:00
URI: http://ir.cftri.com/id/eprint/7667

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