[feed] Atom [feed] RSS 1.0 [feed] RSS 2.0

Response surface modeling of lipase-catalyzed isoamyl propionate synthesis.

Hari Krishna, S. and Karanth, N. G. (2002) Response surface modeling of lipase-catalyzed isoamyl propionate synthesis. Journal of Food Science, 67 (1). 32-36, 17 ref..

[img] PDF
Journal_of_Food_Science_67(1)_2002_32-36.pdf
Restricted to Registered users only

Download (114kB)

Abstract

Response surface methodology based on a 3-variable, 5-level central composite rotatable design matrix was used to optimize the synthesis of the flavour compound isoamyl propionate by esterification of propionic acid using an immobilized lipase (triacylglycerol lipase, EC 3.1.1.3; Lipozyme IM-20 (Novo Nordisk)) from Rhizomucor miehei. The influence of selected variables, namely, enzyme/substrate (E/S) ratio, substrate concn. and temp. was investigated. E/S ratio and temp. had a strong effect on esterification, while substrate concn. did not. Reaction time did not show a significant effect on ester yield, and conversions were completed in <24 h. The response surface model was found to describe experimental results adequately. Product yield at optimum conditions (24 g/mol E/S ratio, 1.5M substrate concn, 20C) was 1.4M, representing 93% conversion; this yield showed good agreement with that predicted by the model.

Item Type: Article
Uncontrolled Keywords: ANALYTICAL-TECHNIQUES; ESTERIFICATION-; ESTERS-; FLAVOUR-COMPOUNDS; IMMOBILIZED-ENZYMES; LIPASES-; PROPIONIC-ACID; RHIZOMUCOR-; ISOAMYL-PROPIONATE; MODELLING-; RESPONSE-SURFACE-METHODOLOGY; RHIZOMUCOR-MIEHEI; TRIACYLGLYCEROL-LIPASES
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 06 Aug 2008 11:01
Last Modified: 28 Dec 2011 09:59
URI: http://ir.cftri.com/id/eprint/7615

Actions (login required)

View Item View Item