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Optimization of the multienzyme system for sucrose biosensor by response surface methodology.

Gouda, M. D. and Thakur, M. S. and Karanth, N. G. (2001) Optimization of the multienzyme system for sucrose biosensor by response surface methodology. World Journal of Microbiology and Biotechnology, 17 (6). 595-600, 11 ref..

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Abstract

An immobilized multienzyme- and cathodic amperometry-based biosensor for sucrose was constructed for analysis of food and fermentation samples. The multienzyme system, comprising invertase (beta-fructofuranosidase; EC 3.2.1.26), mutarotase (aldose 1-epimerase; EC 5.1.3.3) and glucose oxidase (GOD; EC 1.1.3.4), was immobilized using glutaraldehyde as a cross-linking agent. Operating parameters of the biosensor for estimation of sucrose in the range 1-10% were standardized. Response surface methodology based on a three-factor, three-variable design was used to evaluate effect of enzyme activity (10-50 IU invertase, 5-105 IU mutarotase and 1-9 IU GOD) on response of the biosensor. For the range of parameters studied, response time decreased with decreasing invertase and increasing mutarotase and GOD activities. Mutarotase at >75 IU increased the response time through inhibition by its product beta-D-glucose. Optimum conditions for analysis of sucrose were: invertase 10 IU, mutarotase 40 IU and GOD 9 IU. Under these conditions, predicted and actual experimental response times were 2.26 and 2.35 min, respectively, showing good agreement.

Item Type: Article
Uncontrolled Keywords: ANALYTICAL-TECHNIQUES; BIOSENSORS-; GLYCOSIDASES-; IMMOBILIZED-ENZYMES; ISOMERASES-; OXIDASES-; SUCROSE-; beta-FRUCTOFURANOSIDASES; ALDOSE-1-EPIMERASES; GLUCOSE-OXIDASES; RESPONSE-SURFACE-METHODOLOGY
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 31 Sugar Chemistry
600 Technology > 02 Engineering & allied operations
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 30 Jun 2009 09:12
Last Modified: 28 Dec 2011 09:59
URI: http://ir.cftri.com/id/eprint/7614

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