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Optimization of culture conditions for growth of the green alga Haematococcus pluvialis.

Sarada, R. and Sila, Bhattacharya and Ravishankar, G. A. (2002) Optimization of culture conditions for growth of the green alga Haematococcus pluvialis. World Journal of Microbiology and Biotechnology, 18 (6). 517-521, 19 ref..

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Abstract

Effects of light intensity, inoculum vol., sodium nitrate and CO2 concn. on growth of Haematococcus pluvialis were investigated using response surface methodology with the aim of developing a 2-stage technology to produce the food colorant, astaxanthin. All 4 variables had marked effects on growth and were correlated (r <greater than or equal to> 0.926, P <less than or equal to> 0.01) by a 2nd-order polynomial consisting of linear, quadratic and interaction terms. The total quadratic effect (P <less than or equal to> 0.01) dominated over the total linear effect (P <less than or equal to> 0.01) but the role of interaction terms (P <less than or equal to> 0.10) was marginal. Optimum values for variables were: CO2, 1.54%; sodium nitrate, 1.06 g/l; inoculum vol., 24.97%; and light intentisy 2.42 klux. The predicted max. biomass yield was 0.51 g/l (dry wt.).

Item Type: Article
Uncontrolled Keywords: ALGAE-; BIOMASS-; CARBON-DIOXIDE; CAROTENOIDS-; FERMENTATION-TECHNOLOGY; LIGHT-; NITRATES-; ASTAXANTHIN-; CO2-; HAEMATOCOCCUS-PLUVIALIS
Subjects: 500 Natural Sciences and Mathematics > 10 Plants > 04 Plant Physiology
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
Divisions: Grain Science and Technology
Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 30 Jun 2009 09:20
Last Modified: 03 Jul 2015 12:03
URI: http://ir.cftri.com/id/eprint/7600

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