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Isolation and characterization of an extracellular polysaccharide from Pseudomonas caryophylli CFR 1705.

Sudhamani, S. R. and Tharanathan, R. N. and Prasad, M. S. (2004) Isolation and characterization of an extracellular polysaccharide from Pseudomonas caryophylli CFR 1705. Carbohydrate Polymers, 56 (4). pp. 423-427.

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Abstract

A major exopolysaccharide produced during growth of Pseudomonas caryophylli CFR 1705 on lactose-containing medium was isolated and subjected to preliminary characterization. After purification by DEAE-cellulose chromatography, the polysaccharide was found to have a mol. wt. of 1.1 x 106 Da and contained rhamnose, mannose and glucose in respective proportions 1:3.26:4.97. GC MS after methylation demonstrated that the polysaccharide was a highly-branched 1,4-linked hexosan, containing glucose and mannose as branch-off residues and rhamnose as a non-reducing terminal residue.

Item Type: Article
Uncontrolled Keywords: POLYSACCHARIDES-; PSEUDOMONAS-; PURIFICATION-; STRUCTURE-
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry
Divisions: Food Microbiology
Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 21 Nov 2008 05:49
Last Modified: 21 Nov 2008 05:49
URI: http://ir.cftri.com/id/eprint/7543

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