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Modification of guar galactomannan with the aid of Aspergillus niger pectinase.

Shobha, M. S. and Vishu Kumar, A. B. and Tharanathan, R. N. and Rathna, Koka and Anil Kumar, Gaonkar (2005) Modification of guar galactomannan with the aid of Aspergillus niger pectinase. Carbohydrate Polymers, 62. pp. 267-273.

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Abstract

Pectinase from Aspergillus niger associated with polygalacturonase activity, caused considerable debranching–depolymerization of guar galactomannan. Zymogram analysis further confirmed the action of pectinase on the latter. Optimum activity occurred at pH 5.0 and 50 8 Cand the reaction obeyed Michaelis-Menten kinetics with Km and Vmax values of 3.72 mg mL-1 and 1.852 mmoles min-1 mg-1. Viscometry, GPC and HPLC analyses of the native guar galactomannan showed a molecular weight (Mw) of ~240 kDa with a galactose:mannose (G:M)ratio of 1:1.6, as revealed by GLC analysis, whereas, after enzymatic catalysis for 60 min, the major resultant product had a Mw of ~70 kDa with a G:M ratio of 1:2.8, respectively. The latter had a solubility of w98% in water at ambient temperature (within 30 min) compared to ~60% for native galactomannan. IR spectral analysis revealed differences in the conformation of enzyme catalyzed guar galactomannan. The latter showed better gelling property with xanthan gum compared to native galactomannan. Use of non-specific pectinase for debranching–depolymerization of guar galactomannan is of commercial interest as modified galactomannans find multiple applications as functional food ingredients.

Item Type: Article
Uncontrolled Keywords: ASPERGILLUS; FUNCTIONAL PROPERTIES; GLYCOSIDASES; PECTIC ENZYMES; ASPERGILLUS NIGER; MODIFICATION; POLYGALACTURONASES
Subjects: 600 Technology > 08 Food technology > 31 Food Additives
500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Dept. of Biochemistry
Depositing User: Users 197 not found.
Date Deposited: 01 Jun 2011 05:58
Last Modified: 01 Jun 2011 05:58
URI: http://ir.cftri.com/id/eprint/7519

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