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Studies on lipoxygenase from rice bran.

Shastry, B. S. and Rao, M. R. R. (1975) Studies on lipoxygenase from rice bran. Cereal Chemistry, 52 (5). 597-603, 22 ref..

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Abstract

Unfractionated rice bran extract showed on disc-gel electrophoresis 3 distinct bands with lipoxygenase activity. The major fraction was partially purified by repeated ammonium sulphate fractionation at pH 6.8. The partially purified lipoxygenase was optimally active at pH 8.5 and had a Km of 0.35mM using linoleate as a substrate. It was stable for 15 days at 3-5 degree C. The enzyme activity was not affected by alpha,alpha'-dipyridyl and EDTA but partially by 1,10-phenanthroline. Ferrous and calcium ions were activators but copper ions were inhibitory. The enzyme may require tryptophan residues for activity.

Item Type: Article
Uncontrolled Keywords: RICE-; lipoxygenases activity in rice bran; BRAN-; rice bran, lipoxygenases activity in; LIPOXIDASES-
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
600 Technology > 08 Food technology > 21 Cereals > 01 Rice
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 14 Jul 2016 07:45
Last Modified: 14 Jul 2016 07:45
URI: http://ir.cftri.com/id/eprint/7103

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