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Isolation and characterization of a small molecular weight protein of linseed meal.

Madhusudhan, K. T. and Narendra, Singh (1985) Isolation and characterization of a small molecular weight protein of linseed meal. Phytochemistry, 24 (11). 2507-2509, 25 ref..

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Phytochemistry, Volume 24, Issue 11, 29 October 1985, Pages 2507-2509.pdf - Published Version
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Abstract

A small Mr protein from linseed meal has been isolated by CM-Sephadex chromatography. The protein was found to be homogeneous by the techniques of gel filtration, polyacrylamide gel electrophoresis and ultracentrifugation. It had S20,w value of 1.6S. Amino acid composition of the protein revealed a high amount of glutamic acid, cystine, arginine and glycine. The absorption spectrum of the protein consisted of a peak at 280 nm with a shoulder at 290 nm. The fluorescence emission max. was at 340 nm. The protein contained large amounts of alpha-helix and beta-structure. SDS-PAGE showed the protein to consist of a single polypeptide chain. The Mr estimated by Archibald's method, sedimentation-diffusion method and gel filtration was 17 000, 16 000 and 15 000 resp. Difference spectra studies as a function of pH and temp. showed no variation in the conformation of the protein, probably due to disulphide bridges.

Item Type: Article
Uncontrolled Keywords: ELECTROPHORESIS-; linseed meal proteins, SDS-PAGE characterization of; OILSEEDS-; PROTEIN-PRODUCTS
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
600 Technology > 08 Food technology > 19 Lipids-oils/fats > 01 Oilseeds
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 30 Mar 2016 10:42
Last Modified: 30 Mar 2016 10:42
URI: http://ir.cftri.com/id/eprint/6604

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