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Primary metabolites and organic acid metabolism in apple (Malus sylvestris) fruit callus culture.

Prabha, T. N. and Salimath, P. V. and Patwardhan, M. V. (1990) Primary metabolites and organic acid metabolism in apple (Malus sylvestris) fruit callus culture. Journal of the Science of Food and Agriculture, 51 (3). 381-389, 22 ref..

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Abstract

In vitro culture cells were obtained from 7 different var. of Indian apples (Malus sylvestris L.). The cv. Golden Delicious showed the highest yield of callus tissue followed by Maharaji and American Epirouge. Cultured apple cells exhibited some deviation from apple fruit in primary metabolism as well as primary metabolite profiles. In callus cultures, the pool size of free amino acids and organic acids increased considerably while the free sugar pool decreased drastically compared with apple fruit. There was higher incorporation of 14C acetate, 14C citrate, 14C malate and 14C succinate into the CO2, lipid, protein, carbohydrate and amino acid fractions and lower incorporation into the free sugar fraction in cultured cells compared with the explant. Incorporation of 14CO2 showed a similar trend. Qualitatively, there was some similarity between the callus and explant in free amino acid and sugar profiles and dissimilarity in organic acids. Compounds such as citrate, succinate and fumarate and also some amino acids (methionine, arginine, leucine and proline) were present at higher concn. in callus cultures whereas they were almost absent in the original tissue. There were also differences in carbohydrate and protein profiles of explant and callus as judged by their sugar and amino acid make-up, resp.

Item Type: Article
Uncontrolled Keywords: ACIDS-; APPLES-; CELL-CULTURE; FRUITS-
Subjects: 600 Technology > 01 Medical sciences > 12 Metabolism
600 Technology > 08 Food technology > 24 Fruits > 01 Apple
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 26 May 2016 09:38
Last Modified: 26 May 2016 09:38
URI: http://ir.cftri.com/id/eprint/6395

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