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Potential of Aspergillus oryzae CFTRI 1480 for producing proteinase in high titres by solid state fermentation.

Padmanabhan, S. and Ramana Murthy, M. V. and Lonsane, B. K. (1993) Potential of Aspergillus oryzae CFTRI 1480 for producing proteinase in high titres by solid state fermentation. Applied Microbiology and Biotechnology, 40 (4). 499-503, 22 ref..

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Abstract

Aspergillus oryzae CFTRI 1480, an isolate from a spoiled moist sample of casein, produced 59 105 units of an extracellular proteinase/g dry mouldy bran (DMB) at 72 h in an arbitrarily formulated wheat bran medium in a solid state fermentation system. The enzyme production was significantly affected by mineral salt content and pH of the liquid used for moistening the wheat bran. Enzyme titres were enhanced 1.34-fold with the addition of 0.4% corn starch. Optimization of key parameters, i.e. initial moisture content, age and size of inoculum, increased the enzyme production to 191 869 units/g DMB and reduced fermentation time to 48 h. Such high titres in a simple medium, surpassing most of the literature reports, indicate the industrial importance of the culture. The properties of acetone-precipitated enzyme, viz. the optimum pH of 10.0, >95% activity between pH 7.0 and 10.0, temp. optimum at 55

Item Type: Article
Uncontrolled Keywords: ASPERGILLUS-; ENZYMES-; FUNGI-; PROTEINASES-
Subjects: 500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 03 Fungi
600 Technology > 05 Chemical engineering > 04 Fermentation Technology
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 02 Mar 2018 09:07
Last Modified: 02 Mar 2018 09:07
URI: http://ir.cftri.com/id/eprint/6365

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