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An Efficient Method for Extraction of Astaxanthin from Green Alga Haematococcus pluvialis

Sarada, R. and Vidhyavathi, R. and Usha, D. and Ravishankar, G. A. (2006) An Efficient Method for Extraction of Astaxanthin from Green Alga Haematococcus pluvialis. Journal of Agricultural and Food Chemistry, 54. pp. 7585-7588.

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Haematococcus pluvialis is one of the potent organisms for production of astaxanthin, a high value ketocarotenoid. Astaxanthin is accumulated in thick-walled cyst cells of Haematococcus. The thick cell wall is made up of sporopollenin-like material, algaenan, which hinders solvent extraction of astaxanthin. In the present study, an improved method for extraction of astaxanthin without homogenization of cells is reported. Extractability of astaxanthin from cyst cells was evaluated by treating cells with various solvents and pretreating the cells with organic and mineral acids at 70 °C followed by acetone extraction. Hydrochloric acid treatment facilitated 86-94% extractability of astaxanthin. Treatment time, temperature, and concentration of the acid were found to be critical factors for maximum extractability. The treatment did not affect the astaxanthin ester profile and the treated cells can be preserved until further use.

Item Type: Article
Uncontrolled Keywords: Haematococcus; astaxanthin; total carotenoid; extractability
Subjects: 500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 01 Algae
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 07 Apr 2007
Last Modified: 28 Dec 2011 09:27
URI: http://ir.cftri.com/id/eprint/589

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