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Studies on the conformation of alpha-globulin from Sesamum indicum L. in cationic and nonionic detergents.

Prakash, V. and Nandi, P. K. (1982) Studies on the conformation of alpha-globulin from Sesamum indicum L. in cationic and nonionic detergents. Journal of Biosciences, 4 (3). pp. 331-345.

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Abstract

The circular dichroic spectra of a-globulin from Sesamum indicum L. was recorded in the presence of cetyltrimethyl ammonium bromide, Triton X-100 and Brij-36T. The protein in 0.2 Μ phosphate buffer pH 7.4 had about 25% β-structure and 5% α-helix, the rest being aperiodic or irregular structure and a-helix, structure was increased by cationic detergent cetyl trimethyl ammonium bromide. But, the increase in α-helix content was much less than that induced by an anionic detergent, sodium dodecyl sulphate. In non-ionic detergent like Brij-36T and Triton X-100, specific β-structures like II-β and I-β were formed along with changes in α-helical and aperiodic structures. These results suggest that the protein has a fairly labile quaternary structure.

Item Type: Article
Uncontrolled Keywords: Circular dichroism; α-globulin; cationic detergent; nonionic detergent; dissociation and denaturation; I-β and II-β structures
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
600 Technology > 08 Food technology > 19 Lipids-oils/fats > 01 Oilseeds
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 28 Mar 2018 05:36
Last Modified: 28 Mar 2018 05:36
URI: http://ir.cftri.com/id/eprint/5724

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