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Different biotic and abiotic elicitors influence betalain production in Savithahairy root cultures of Beta vulgaris in shake-flask and bioreactor.

Savitha, B. C. and Thimmaraju, R. and Bhagyalakshmi, N. and Ravishankar, G. A. (2006) Different biotic and abiotic elicitors influence betalain production in Savithahairy root cultures of Beta vulgaris in shake-flask and bioreactor. Process Biochemistry, 41 (1). pp. 50-60. ISSN 1359-5113

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Abstract

The hairy roots of Beta vulgaris grow on a simple medium producing good levels (1.2% or 88.4 mg L<sup>−1</sup>) of betalains—a red water-soluble food colourant. In an attempt to enhance betalain productivity, the hairy roots were contacted with several biotic elicitors such as purified glycans of microbial origin (200–500 mg L<sup>−1</sup>), extracts of whole microbial cultures (0.25–1.25%) and the respective culture filtrates (5–25%, v/v). Similarly, abiotic elicitors, particularly metal ions, upto 10-folds of that present in the nutrient (MS) medium, were tested. It was observed that though there was a significant suppression of biomass in almost all the treatments, a significantly high productivity of betalain was observed in <i>Penicillium notatum</i> DCP-treated cultures (158 mg L<sup>−1</sup> on 7th day) among biotic elicitors, pullulan-treated cultures (202 mg L<sup>−1</sup> on 10th day) among purified glycans and calcium treated cultures (127 mg L<sup>−1</sup> on 7th day) among abiotic elicitors, whereas control cultures showed productivities of only 43.3 mg L<sup>−1</sup> on 7th day and 88.4 mg L<sup>−1</sup> on 10th day. Since most of the elicitors caused early elicitation (on 7th day) and suppressed biomass resulting in reduced overall productivity, a strategy of using elicitor at late exponential growth phase was considered and such a strategy was adoptable to scaled up process using a bubble-column bioreactor, where too the addition of elicitor at late exponential phase resulted in about 47% higher productivity of betalains. The present study is the first report where a large number of elicitors are systematically screened and used for scaled-up production of betalains.

Item Type: Article
Additional Information: Copyright of this article belongs to Elsevier Ltd.
Uncontrolled Keywords: Betalain; Elicitation; Calcium; Culture filtrate; Bubble column reactor
Subjects: 600 Technology > 08 Food technology > 23 Vegetables > 06 Beet Root
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 07 Apr 2007
Last Modified: 28 Dec 2011 09:27
URI: http://ir.cftri.com/id/eprint/567

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