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Studies on groundnut proteins. VI. Isolation, characterisation and hydrogen ion titration of conarachin 11.

Jayarama Shetty, K. and Narasinga Rao, M. S. (1977) Studies on groundnut proteins. VI. Isolation, characterisation and hydrogen ion titration of conarachin 11. International Journal of Peptide and Protein Research, 9. pp. 11-17.

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Abstract

A method is described for isolating conarachin II in a homogeneous form by the techniques of DEAE-cellulose chromatography, polyacrylamide gel electrophoresis and sedimentation velocity. The protein contains 0.72% carbohydrate and no phosphate. Hydrogen ion titration curve indicated that the sidechain carboxyl, imidazole and €-amino groups titrated with normal pKInt values and their number agreed with the analytical values obtained from amino acid analysis. However, tyrosine phenolic groups had abnormal pKint of 10.5.

Item Type: Article
Uncontrolled Keywords: conarachin II, proteins groundnut, Arachis hypogaea, hydrogen ion
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
600 Technology > 08 Food technology > 19 Lipids-oils/fats > 05 Nuts
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 10 Feb 2015 05:58
Last Modified: 10 Feb 2015 05:58
URI: http://ir.cftri.com/id/eprint/5626

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