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Structural characteristics of a mixed linkage Beta-D-glucan from sorghum (Sorghum bicolor).

Ramesh, H. P. and Tharanathan, R. N. (1998) Structural characteristics of a mixed linkage Beta-D-glucan from sorghum (Sorghum bicolor). Carbohydrate Research, 308. pp. 239-243.

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Abstract

Hemicellulose B, obtained by alkali extraction of sorghum ¯our on DEAE-cellulose chromato- graphy gave a water-eluted neutral polysaccharide composed exclusively of d-Glc. Further pur- i®cation on Sephacryl S-400 gave a major fraction (Fra.II) which was homogeneous by electrophoretic and SEHPLC methods. It had a MW of 3.6�104 Da and showed a bathochromic shift (19nm) in the lmax of its complex with Congo Red. Permethylation and GC±MS analysis revealed (1!4)- and (1!3)-glucosidic linkages in a ratio of 2.3:2 which were corroborated by 13C NMR and CD spectral measurements. The � con®guration of the glucan was further con®rmed by treatment with �-glucanase and also by its low [�]D value of +12.5�.

Item Type: Article
Uncontrolled Keywords: Sorghum; Hemicellulose; Mixed linkage �-d-glucan; Structural studies
Subjects: 600 Technology > 08 Food technology > 21 Cereals > 06 Sorghum
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 21 Nov 2008 06:31
Last Modified: 28 Dec 2011 09:50
URI: http://ir.cftri.com/id/eprint/5521

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