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Standardisation of assay procedure and some properties of ribonuclease from Aspergillus candidus.

Mohammad Kunhi, A. A. and Singh, R. (1981) Standardisation of assay procedure and some properties of ribonuclease from Aspergillus candidus. Folia Microbiologica, 26 (4). pp. 328-333.

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Abstract

Screening of several fungal cultures resulted in the selection of an isolate of Aspergillus candidus which produced a considerable amount of RNA-degrading enzyme in both surface and submerged methods of cultivation. The conditions for the assay of the RNAase were standardized at pH 4.5, 55 ~ and using 0.25 ~o yeast RNA as substrate. The enzyme was stable at pit 5.2. EDTA was found to activate the enzyme slightly. At temperatures 50-- 60 ~ there was considerable loss in enzyme activity which was traced to the presence of a contaminating protease which presumably degraded the RNAase optimally at this temperature. The protease could be preferentially inactivated at or above 75 ~ The crude enzyme, in addition to RNAaso was found to possess DNAase, nonspecific phosphodiesterase and 3'- and 5'-phosphomonoesterase activities.

Item Type: Article
Uncontrolled Keywords: Aspergillus candidus, Ribonuclease enzymes, RNAase
Subjects: 500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 03 Fungi
600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 05 Mar 2018 08:51
Last Modified: 05 Mar 2018 08:51
URI: http://ir.cftri.com/id/eprint/5477

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