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Spectrofluorodensitometric estimation in thin layer chromatography of gibberellic acid produced by solid state fermentation.

Kumar, P. K. R. and Lonsane, B. K. (1986) Spectrofluorodensitometric estimation in thin layer chromatography of gibberellic acid produced by solid state fermentation. Journal of Chromatography, 369. pp. 222-226.

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Abstract

Gibberellic acid, an important plant growth regulator, is used extensively in agriculture, nurseries, green houses, viticulture, tea-gardens, etc. l. It is traditionally produced by submerged fermentation by employing Gibberella fujikuroi or Fusarium monol~forme*. Spectrophotometric, fluorometric and bioassay methods are routinely used for the estimation of gibberellic acid in fermentation broths or in crude forms3 5, though a variety of other methods are available for its estimation6-lo. Recently, the potential of solid-state fermentation for production of gibberellic acid was established’ l. Preliminary studies indicated that the above routine analytical methods give poor responses unless the crude extract of mouldy bran obtained by solid-state fermentation is extensively purified. Since microbial products, such as mycotoxinsr2-‘*, can now be estimated by spectrofluorodensitometry with greater accuracy19 without resorting to extensive purification, we studied the application of this technique to the estimation of gibberellic acid in crude extracts of mouldy bran obtained by solidstate fermentation.

Item Type: Article
Uncontrolled Keywords: Gibberellic acid, Gibberella fujikuroi, Fusarium monol~forme, Spectrophotometric, fluorometric, bioassay methods
Subjects: 600 Technology > 08 Food technology > 20 Plant Food
600 Technology > 05 Chemical engineering > 03 Enzyme Biotechnology and Engineering
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 14 Jun 2011 11:17
Last Modified: 28 Dec 2011 09:50
URI: http://ir.cftri.com/id/eprint/5454

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