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Silver nitrate influences in vitro root formation in Decalepis hamiltonii wight and Arn.

Harsh Pal, Bais and Sudha, G. and Suresh, B. and Ravishankar, G. A. (2000) Silver nitrate influences in vitro root formation in Decalepis hamiltonii wight and Arn. Current Science, 79. pp. 894-898.

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Abstract

Effects of AgNO3 on root formation were examined in vitro using axillary bud cultures of Decalepis hamiltonii. In the case of a medium comprising of MS salts with indole 3-acetic acid (IAA) (0.5 mg l–1), poor rooting was observed and root emergence did not occur until after 25 days. The resultant roots were stunted. Addition of 40 μM AgNO3 improved root initiation and elongation. The promotive effects of AgNO3 on rooting may result from inhibition of ethylene action. Upon addition of ethephon to the rooting medium, excessive callusing was observed in explants in all the treatments. Addition of 40 μM AgNO3 to ethephon-containing medium resulted in improvement in root initiation and elongation. Ethylene production was monitored in all the treatments with IAA/AgNO3/ethephon and it was observed that the treatment with IAA (0.5 mg l–1) alone showed a greater increase in ethylene production when compared with AgNO3, ethephon and their combinations.

Item Type: Article
Uncontrolled Keywords: axillary bud cultures, plant tissue culture, Nicotiana plumbaginifolia
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 06 Jun 2011 09:43
Last Modified: 18 Nov 2016 11:11
URI: http://ir.cftri.com/id/eprint/5351

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