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Heteronuclear saturation transfer difference (HSTD) experiment for detection of ligand binding to proteins.

Nagaraja, C.S. (2006) Heteronuclear saturation transfer difference (HSTD) experiment for detection of ligand binding to proteins. Chemical Physics Letters, 420 (4-6). pp. 340-346. ISSN 0009-2614

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Abstract

This report presents a modified saturation transfer difference experiment for protein–ligand binding studies. A heteronuclear saturation transfer difference (HSTD) is suggested, where in a hetero atom, such as carbon is utilized for monitoring the binding instead of proton. This method is free from some of the problems associated with proton STD experiment, such as lack of sufficient number of protons at the binding site or crowding of spectra due to smaller chemical shift dispersion. The present method has been demonstrated on three systems namely caffeine–HSA, salicylic acid–HSA and glucose–lysozyme, illustrating the utility of the method.

Item Type: Article
Additional Information: Copyright of this article belongs to Elsevier.
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry
Divisions: Plantation Products Spices and Flavour Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 26 Apr 2007
Last Modified: 28 Dec 2011 09:27
URI: http://ir.cftri.com/id/eprint/535

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