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Fermentative Production of Beta-galactosidase from ENTEROCOCCUS FAECIUM MTCC 5153

Nandhini, E. (2006) Fermentative Production of Beta-galactosidase from ENTEROCOCCUS FAECIUM MTCC 5153. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: E.faecium is considered as an important organism for dairy industry. In dairy industry, b-galactosidase enzyme is rather used as crude extract to prevent high cost of purification. Thus it is important to characterize the enzyme in crude extract of b-galactosidase producers.The influence of different medium on growth and b-Gal production by Ent.faecium was studied. After identifying the lactose fermenting capacity of strain Enterococcus faecium MTCC 5153 it was decided to explore lactose-based medium for the production of b-galactosidase. Three media were used by substituting the carbon source for the production of b-galactosidase. It was found that maximum cell biomass was obtained in MRS broth. In another experimental trial, low-cost TGE was tested. It was found that glucose do not induce b-galactosidase production so negligible activity was observed. Glucose of TGE was substituted with lactose and TYE was formulated which showed a very high titre of b-galactosidase production. Based on the above results and the utilization of lactose as a carbon source, production of b-galactosidase in whey permeate (WP) was studied. Normal cell growth and a high titre of b-galactosidase were obtained but less than TYE. In our experiment to facilitate healthy cell growth, 6ml of WP was supplemented with 2% YE. Ent. faecium showed maximum activity when grown in the presence of 2% lactose, followed by skim milk. But no b-galactosidase activity appeared when it is grown in the presence of glucose.
Uncontrolled Keywords: E.faecium beta-galactosidase Fermentative Production
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 31 Sugar Chemistry
600 Technology > 05 Chemical engineering > 04 Fermentation Technology
600 Technology > 08 Food technology > 09 Food Microbiology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 20 Mar 2007
Last Modified: 28 Dec 2011 09:27
URI: http://ir.cftri.com/id/eprint/493

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