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Multiple forms of aspartate amino transferase from germinated green gram (Phaseolus aureus Roxb.).

Lalitha, K. and Patwardhan, M. V. and Radhakrishnamurthy, R. (1974) Multiple forms of aspartate amino transferase from germinated green gram (Phaseolus aureus Roxb.). Indian Journal of Biochemistry and Biophysics, 11. pp. 216-220.

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Abstract

L-Aspartate 2-oxoglutarate aminotransferases (EC 2.6.1.1), one from the supernatant and three from the mitochondrial extract of 3-day old seedlings of green gram, have been purified and some of their properties studied. The major supernatant enzyme (200-foid purified) moved as a singie band of en~yme activity on polyacrylamide gel electrophoresis. All the partially purified enzymes were anionic as shown by agar gel electrophoresis at pH 7·4. Kinetic studies indicated a binary mechanism being operative. Data obtained on pH pattern, Km vaiues, eiectrophoretIc and chromatographic studies· suggest isoenzymic nature of these enzyme forms.

Item Type: Article
Uncontrolled Keywords: green gram, aspartate aminotransferases
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 07 Enzyme Biochemistry
600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 20 Sep 2013 09:09
Last Modified: 20 Sep 2013 09:09
URI: http://ir.cftri.com/id/eprint/4673

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