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Mineralisation of phenol and its derivatives by Pseudomonas sp. strain CP4.

Babu, K. S. and Ajith Kumar, P. V. and Kunhi, A. A. M. (1995) Mineralisation of phenol and its derivatives by Pseudomonas sp. strain CP4. World Journal of Microbiology and Biotechnology, 11 (6). pp. 661-664.

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Abstract

A Pseudomonas sp. strain, CP4, was isolated that used phenol up to 1.5 g/l as sole source of carbon and energy. Optimal growth on 1.5 g phenol/l was at pH 6.5 to 7.0 and 30°C. Unadapted cells needed 72 h to decrease the chemical oxygen demand (COD) of about 2000 mg/l (from 1 g phenol/l) to about 200 mg/l. Adapted cells, pregrown on phenol, required only 65 h to decrease the COD level to below 100 mg/l. Adaptation of cells to phenol also improved the degradation of cresols. Cell-free extracts of strain CP4 grown on phenol or o-, m- or p-cresol had sp. act. of 0.82, 0.35, 0.54 and 0.32 units of catechol t,d-dioxygenase and 0.06, 0.05, 0.05 and 0.03 units of catechol l,&dioxygenase, respectively. Cells grown on glucose or succinate had neither activity. Benzoate and all isomers of cresol, creosote, hydroxybenzoates, catechol and methyl catechol were utilized by strain CP4. No chloroaromatic was degraded, either as sole substrate or as co-substrate.

Item Type: Article
Uncontrolled Keywords: Catechol2,&dioxygenase, cresols, mineralization, phenol, Pseudomonas sp.
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 19 Apr 2012 04:53
Last Modified: 19 Apr 2012 04:53
URI: http://ir.cftri.com/id/eprint/4647

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