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L-galactosidase from germinating guar (Cyamopsis tetragonolobus) seeds.

Shivanna, B. D. and Ramakrishna, M. (1985) L-galactosidase from germinating guar (Cyamopsis tetragonolobus) seeds. Journal of Biosciences, 9 (1-2). pp. 109-116.

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Abstract

The changes in alpha galactosidase activity in guar (Cyamopsis tetragonolobus) seeds was followed during seven days of germination. The enzyme activity was maximal on the first day of germination and gradually decreased during subsequent days. On the second day of germination the partially purified enzyme upon ion-exchange chromatography on CMSephadex C-50 was resolved into a-galactosidase-A (anionic). alpha galactosidase-C1 (cationic) and a-galactosidase-C. (cationic) and their relative proportions were 28, 12 and 60~~~, respectively. The combined alpha galactosidase C1 and C1 activities increased in the first two days of germination followed by significant decrease after the 3rd day onwards, whereas alpha galactosidase ·A remained fairly constant throughout the germination period, alpha Galactosidase- A and C2 had different Km and Vmax values with p-nitrophenyl alpha galactopyranoside, raffinose and melibiose as substrates and also differed in their thermal stabilities.

Item Type: Article
Uncontrolled Keywords: Guar, Cyamopsis tetragonolobus; Leguminoseae, germination; alpha-galactosidase
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 07 Enzyme Biochemistry
600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 23 Jun 2016 05:30
Last Modified: 23 Jun 2016 05:30
URI: http://ir.cftri.com/id/eprint/4536

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