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Isolation, purification and some properties of hemicellulase from Fusarium

Wankhede, D. B. and Vijayalakshmi, K. R. and Raghavendra Rao, M. R. (1981) Isolation, purification and some properties of hemicellulase from Fusarium. Carbohydrate Research, 96. pp. 249-257.

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Abstract

An extracellular hemicellulase from a soil fungus (Fusarium sp.) grown on a medium containing groundnut hemicellulose B was purified 76-foId by ammonium sulphate fractionation, chromatography on DEAE-cellulose, and gel filtration on Sephadex G-100. It was found to be homogeneous by disc-gel electrophoresis at pH 8. It showed optimal activity at pH 5.6 and 37”. It was observed that groundnut and sesame hemicellulose B were degraded considerably (m 80 and 58 %, respectively) by the purified hemicellulase, whereas glucomannan and xylan from groundnut were comparatively poorly hydrolysed (N 30-40 %)_

Item Type: Article
Uncontrolled Keywords: Hemicellulases, bacteria, fungi, insects, plant materials
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 08 Jun 2011 09:17
Last Modified: 28 Dec 2011 09:47
URI: http://ir.cftri.com/id/eprint/4525

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