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Estimation of vitamin B12 (hydroxocobalamin) in proteolysed liver extracts.

Sreenivasamurthy, V. and Swaminathan, M. and Subrahmanyan, V. (1956) Estimation of vitamin B12 (hydroxocobalamin) in proteolysed liver extracts. Journal of Scientific and Industrial Research, 15C. pp. 215-217.

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The stability of hydroxocobalamin to ascorbic acid-sodium ascorbate (AAM) in the presence of varying concentrations of iron has been studied. Iron In concentrations higher than 3-5 ['g./ml. prevents the destruction of hydroxocobalamin by AAM to varying, extents, 100 per cent protection being, attained at 32 l~g,. level. At levels less than 1·5 ['g., iron does not exhibit any protective action. Cyanocobalam.in is not destroyed under the above conditions. Hydroxocobalamin present in proteolysed liver extracts couId be selectively destroyed by treatment with AAM if the iron content of the extract is adjusted to about 1 ['g./ml. by suitable dilution of the extract. Based on the above findings, a method for the determination of hydroxocobalamin content of liver extracts has been developed. The results of analysis of a few samples of proteolysed liver extracts indicate that about 50-60 per cent of the vitamin B,. activity of the extracts is due to hydroxocobalarnill.

Item Type: Article
Uncontrolled Keywords: vitamin B12, hydroxocobalamin, proteolysed liver extracts
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 04 Vitamins
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 11 Mar 2015 07:53
Last Modified: 11 Mar 2015 07:53
URI: http://ir.cftri.com/id/eprint/4142

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