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Improved Chromatographic Method for the Purification of Phenolic Constituents of the Lichen Parmotrema tinctorum (Nyl.) Hale.

Jayaprakasha, G. K. and Jaganmohan Rao, L. and Singh, R.P. and Sakariah, K. K. (1998) Improved Chromatographic Method for the Purification of Phenolic Constituents of the Lichen Parmotrema tinctorum (Nyl.) Hale. Journal of Chromatographic Science, 36. pp. 8-10. ISSN 0021-9665

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Abstract

A method for the separation and fractionation of the phenolic compounds atranorin, methyl orsenillate, orsenillic acid, and lecanoric acid from the lichen Parmotrema tinctorum (Nyl.) Hale (P. tinctorum) using thin-layer chromatography (TLC) and column chromatography, respectively, is described. TLC of lichen extract with an acid-free developing solvent on silica gel G containing 1 % oxalic acid is carried out, and the compounds are visualized as yellow spots when sprayed with 10% sulfuric acid in methanol followed by heating at 110°C. For the fractionation of pure lichen compounds, column chromatography is carried out using silica gel impregnated with 1 % oxalic acid. To our knowledge, it is also the first report on the isolation and characterization of methyl orsenillate and orsenillic acid from P. tinctorum (Nyl.) Hale.

Item Type: Article
Uncontrolled Keywords: Thin-layer chromatography, lichen compounds, Parmotrema tinctorum
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 06 Chromatography
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
Divisions: Human Resource Development
Plantation Products Spices and Flavour Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 20 Feb 2012 04:46
Last Modified: 20 Feb 2012 04:46
URI: http://ir.cftri.com/id/eprint/4090

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