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Regulation of the Expression of PHA synthase from Pseudomonas aeruginosa using antisense technology

Bibha, Kumari (2005) Regulation of the Expression of PHA synthase from Pseudomonas aeruginosa using antisense technology. Masters thesis, University of Mysore.

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Thesis (Masters)
Additional Information: <p align="justify">The present work was based on the utilization of antisense technology to regulate the expression of PHA synthase gene from Pseudomonas aeruginosa.The present antisensing work provides evidence for the differential regulation/expression of PHA synthase in glucose and octanoate media as demonstrated by earlier workers (Chen, et al., 2004) The results obtained in this experiment supports the concept that both phaC1and phaC2 have separate promoter, specific function and are not interchangeable. It is a better tool to determine the function of a gene rather than gene disruption because in later technique there is complete silencing of gene, which has its own limitations while antisensing provides degree of silencing. This work is also showing deviation in the composition of PHA from the native one (non-recombinant) so this technique can be used in production of modified PHA. Promoter antisensing is a novel and unique approach, which can be used in the development of ribo-switches for better control on gene expression.The use of promoter for silencing the expression of a gene requires further confirmation.</p>
Uncontrolled Keywords: PHA poly hydroxy alkanoates Pseudomonas aeruginosa antisense technology
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Human Resource Development
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 23 Feb 2007
Last Modified: 28 Dec 2011 09:26
URI: http://ir.cftri.com/id/eprint/401

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