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Effect of sodium dodecyl sulfate, acid, alkali, urea and guanidine hydrochloride on the circular dichroism of alpha-globulin of Sesamum indicum L.

Prakash, V. and Nandi, P. K. and Jirgensons, B. (1980) Effect of sodium dodecyl sulfate, acid, alkali, urea and guanidine hydrochloride on the circular dichroism of alpha-globulin of Sesamum indicum L. International Journal of Peptide and Protein Research, 15. pp. 305-313.

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Abstract

The circular dichroic spectra of a-globulin have been obtained under various solution conditions of sodium dodecyl sulfate, acid, alkali, urea and guanidine hydrochloride. The protein in phosphate buffer pH 7.4, 0 . 2 , h~a s about 25% bstructure and 5% a-helix, the rest being aperiodic or irregular structure. Sodium dodecyl sulfate induced more a-helical structure in the protein. The protein had nearly 20% a-helix at 1 x lo-= M SDS. At extreme acid or alkaline pH, the protein had no a-helix with Pstructure decreasing with further extremes of pH. The protein is represented by 100% aperiodic structure in 6.6 m urea and in 6.0 m guanidine hydrochloride solutions. The above results are discussed in view of some of the earlier results with regard to the association-dissociation and denaturation behavior of a-globulin under various solution conditions.

Item Type: Article
Uncontrolled Keywords: acid; alkali; aperiodic structure; association; circular dichroism; denaturation; dissociation; a-globulin; GuHCl; a-helix; SDS; beta-structure; subunits; urea
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
600 Technology > 08 Food technology > 19 Lipids-oils/fats > 01 Oilseeds
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 13 Mar 2018 06:35
Last Modified: 13 Mar 2018 06:35
URI: http://ir.cftri.com/id/eprint/4006

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