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PCR cloning of phaC gene of Alcaligenes latus MTCC 2309 and PHA production aspects

Gloria, Lovsey (2005) PCR cloning of phaC gene of Alcaligenes latus MTCC 2309 and PHA production aspects. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: PHA synthase is the most interesting enzyme of the PHA biosynthetic pathway, since the substrate specificity of this enzyme determines the molecular composition of the polyester. The gene, which codes for PHA synthase enzyme is the pha C gene. In this study, the pha C gene of A. latus MTCC 2309 (1.6 Kb long) was cloned in pTZ vector. The pTZ is a T-tailed vector with a size of 2.8 Kb which provides an efficient system for cloning of PCR product generated by Taq polymerase. This recombinant was then transformed in E. coli DH5α strain. PHA is gaining importance nowadays because of its wide range of applications but since the production cost is high, cheaper carbon sources are preferred. Lactose, a cheap carbon source, is used in this study for PHA production. The PHA polymer produced by PHA producing A. latus and Bacillus cereus ‘CFR04’ strain grown in lactose was analyzed by GC and FTIR. The main objectives were:- Cloning of pha C of A. latus in pTZ vector. Analysis of PHA of A. latus and Bacillus cereus grown in 1 and 2% lactose.
Uncontrolled Keywords: Alcaligenes latus PHA synthase PHAs
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 22 Dec 2006
Last Modified: 28 Dec 2011 09:26
URI: http://ir.cftri.com/id/eprint/364

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