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Comparison of ELISA and GC methods to detect DDT residues in water samples.

Amita Rani, B. E. and Akmal, Pasha. and Putte, Gowda. and Nagendraprasad, T. R. and Karanth, N. G. K. (2002) Comparison of ELISA and GC methods to detect DDT residues in water samples. Indian Journal of Biotechnology, 1 (3). 292-297, 11 ref..

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ELISA and GC methods were used to analyse DDT residues in approx. 30 water samples collected from different sources in the Mandya district of Karnataka, India. The polyclonal antibody based immunoassay performed well in detecting the DDT residues. The min. detectable level of DDT by ELISA was 1 ppb in the water samples tested. Levels of insecticide residues detected ranged from 1 to 20 ppb, and the assay was specific for DDT. Experiments also revealed no matrix effect and hence did not require any prior clean-up. The pH of the water did not interfere in the assay. The results of ELISA with respect to DDT residues were comparable to values obtained from the GC analysis of the water samples. It is suggested that the water samples could be tested directly using the ELISA test, thereby making the analysis quick, simple and cost effective.

Item Type: Article
Subjects: 600 Technology > 08 Food technology > 12 Allergy & Toxicology
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry
Divisions: Food Protectants and Infestation Control
Depositing User: Somashekar K.S
Date Deposited: 18 Jul 2011 04:50
Last Modified: 20 Feb 2012 10:59
URI: http://ir.cftri.com/id/eprint/3577

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