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Colorimetric determination of sinalbin (sinapin p-hydroxybenzylglucosinolate).

Raghavan, B. and Shankaranarayana, M. L. and Nagalakshmi, S. and Natarajan, C. P. (1972) Colorimetric determination of sinalbin (sinapin p-hydroxybenzylglucosinolate). Mikrochimica Acta, 6. pp. 818-822.

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Abstract

The determination of sinalbin in white mustard seed via the product of enzymic hydrolysis, p-hydroxybenzyl isothiocynate (p-HBI), is described. Reaction of p-HBI with NaOH produces p-hydroxybenzyl alcohol and NaCNS, which is converted into CNBr and treated with pyridine-benzidine reagent. Resulting colour is linear at 518 nm between 0.1 and 0.5 mug CNS/ml. 1 mole sinalbin produces 1 mole CNS-- with a recovery of 90%.

Item Type: Article
Uncontrolled Keywords: MUSTARD-; sinalbin detn. in mustard seed
Subjects: 600 Technology > 08 Food technology > 01 Analysis
600 Technology > 08 Food technology > 19 Lipids-oils/fats > 04 Mustard seed
Divisions: Plantation Products Spices and Flavour Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 20 Dec 2016 07:20
Last Modified: 16 Jan 2017 13:20
URI: http://ir.cftri.com/id/eprint/3515

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