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Structural Studies of the Proteinase Inhibitors of Germinated Horsegram(Dolichos Biflorus) Seeds

Pradeep, Kumar (2004) Structural Studies of the Proteinase Inhibitors of Germinated Horsegram(Dolichos Biflorus) Seeds. PhD thesis, University of Mysore.

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Abstract

The evolutionary relationship among various BBI isoforms of horsegram was studied. Several inhibitor isoforms have been purified from seeds, cotyledons, leaves and flowers of horsegram and characterized. The data compiled in this thesis describe the purification and characterization of Bowman-Birk inhibitors from various tissues of horsegram (Dolichos biflorus) plant. The self-association, an anomalous behavior of legume BBIs was analyzed by comparing the primary structure of HGGI-III and HGI-III. The crucial interaction between Lys24 at P1 of the first reactive site of one subunit and the charged residue Asp76 at the carboxy-terminal of another subunit is the premise of selfassociation in HGI-III. Comparative studies of legume BBI sequences and their self-association behavior indicated that similar interaction could be responsible for the self-association of all reported legume BBIs. Lack of highly immunogenic amino- and carboxy-terminal residues, low Mr and high potency of HGGIs could be an excellent alternative for use as a cancer chemopreventive agent and developing transgenic insect resistant plants.

Item Type: Thesis (PhD)
Uncontrolled Keywords: Horsegram Horsegram seeds Proteinase Inhibitors
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry
500 Natural Sciences and Mathematics > 10 Plants
600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 20 Dec 2006
Last Modified: 28 Dec 2011 09:26
URI: http://ir.cftri.com/id/eprint/351

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