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Binding of Mg(II) by the 11S fraction of soybean proteins.

Rao, A. G. A. and Rao, M. S. N. (1975) Binding of Mg(II) by the 11S fraction of soybean proteins. Journal of Agricultural and Food Chemistry, 23 (4). 657-661, 16 ref..

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Abstract

Binding of Mg(II) by the 11s fraction of soybean proteins has been measured in 0.1 M borate buffer of pH 7.8 by equilibrium dialysis. The binding sites are possibly the imidazole groups of the histidine residues of the protein molecule. NaCl at 0.5 M concentration suppresses binding. Addition of utea or .prior treatment of the protein with EDTA reduces binding, EDTA treatment causes dissociation of the proteiD which is partly re-versed by the addition of Mg(II). No confonnational change occurs in the protein due to the binding of Mg(II). The protein, both before and after EDTA treatment, is quantitatively precipitated by 1 x 10-2 M Mg(II). NaCI at 0.5 M concentration suppresses precipitation. Addition of Mg(II) to tbe water extract of defatted soybean yields a precipitate which consists almost entirely of the 11S fraction.

Item Type: Article
Uncontrolled Keywords: 11s soybean proteins, Mg(II)binding
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
600 Technology > 08 Food technology > 22 Legumes-Pulses > 05 Soya bean
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 14 Jul 2016 07:54
Last Modified: 14 Jul 2016 07:54
URI: http://ir.cftri.com/id/eprint/3156

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