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Isolation and identification of a radical scavenging antioxidant – punicalagin from pith and carpellary membrane of pomegranate fruit

Kulkarni, Anand. P. and Aradhya, S. M. and Divakar, S. (2004) Isolation and identification of a radical scavenging antioxidant – punicalagin from pith and carpellary membrane of pomegranate fruit. Food Chemistry, 87. pp. 551-557. ISSN 0308-8146

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Abstract

<p align="justify">Pith and carpellary membrane (CM) constitute 13% of pomegranate fruit composition. Pith acts as an anchoring tissue while CM acts as a protective membrane; both play significant roles in determining the health and disease status of pomegranate arils but remain unexplored for their nutraceutical properties. Activity-guided repeated fractionation of the methanol extract on a silica gel column, followed by preparative HPLC, yielded a compound which exhibited strong radical scavenging activity with EC<sub>50</sub> values of 16.7 plus or minum 2.3 and 54.2 plus or minus 4.6 against DPPH free radical and superoxide radicals, respectively. It also showed a very strong lipid peroxidation inhibitory activity in a liposome model system with an EC<sub>50</sub> value of 54.2 plus or minus 0.9. However, it showed negligible metal chelating activity. Based on UV, IR, 2D-NMR, GC-Mass and MALDI-Mass studies, the compound was identified as punicalagin. The antioxidant activity of punicalagin and the methanol extract can be ascribed mainly to radical scavenging ability.</p>

Item Type: Article
Uncontrolled Keywords: Punica granatum; Antioxidant; Isolation; Identification; Pith and carpellary membrane; Punicalagin
Subjects: 600 Technology > 08 Food technology > 24 Fruits
Divisions: Fruit and Vegetable Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 07 Jun 2005
Last Modified: 14 Dec 2016 10:43
URI: http://ir.cftri.com/id/eprint/31

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