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Antifungal proteins from sorghum endosperm and their effects on fungal mycelium.

Sunitha Kumari, R. and Chandrashekar, A. and Shetty, H. S. (1994) Antifungal proteins from sorghum endosperm and their effects on fungal mycelium. Journal of the science of Food and Agriculture, 66 (2). pp. 121-127.

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Abstract

Three antifungal proteins identified in sorghum endosperm act on the grain mould pathogen Fusarium moniliforme Sheldon causing inhibition of growth (Sunitha Kumari and Chandrashekar J Sci Food Agric 64 (1994) 357- 364). Both morphological and biochemical changes in the fungal hyphae were observed on treatment with the antifungal proteins. The 18 kDa antifungal protein caused sloughing of cell wall polysacharides without much release of cytoplasmic material as seen by a slight increase in absorbance at 280 and 265 nm, and by microscopic examination. The decrease in pH or rise in electrical potential of the medium as the fungi respired was not altered by the presence of the 18 kDa antifungal protein in the incubation mixture. The 26 and 30 kDa protein fraction on the other hand caused leakage of cytoplasmic contents as observed microscopically without releasing polysaccharides from the cell wall. Addition of 26 and 30 kDa proteins to the incubation medium reduced the rate of the rise in pH or fall in electrical potential of the medium. Immunofluorescent microscopy indicated that the 18 and 26 kDa proteins bound to discrete areas of the fungal hyphal walls whereas, the binding of the 30 kDa antifungal protein was non-specific.

Item Type: Article
Uncontrolled Keywords: sorghum endosperm, antifungal proteins, Fusarium moniliforme
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
600 Technology > 08 Food technology > 21 Cereals > 06 Sorghum
Divisions: Food Microbiology
Grain Science and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 08 May 2012 05:39
Last Modified: 08 May 2012 05:39
URI: http://ir.cftri.com/id/eprint/3039

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