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Anaerobic degradation of pectin by mixed consortia and optimization of fermentation parameters for higher pectinase activity.

Shivakumar, P and Krishna, Nand. (1995) Anaerobic degradation of pectin by mixed consortia and optimization of fermentation parameters for higher pectinase activity. Letters in Applied Microbiology, 20 (2). 117-119, 15 ref..

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Abstract

Samples from biogas digesters, sewage ponds, animal house effluents and food processing wastes were used in enrichment systems seeking anaerobic bacteria producing pectinases. Among the 46 anaerobic consortia developed from various samples, four showed high pectinase activity under static anaerobic conditions. Investigation of fermentation variables showed the optimum conditions for pectinase activity were pH 7*0,45"C and 72 h of growth with 0.5% pectin in the cultivation medium. A 1.4- to 1 -6-fold increase in the pectinase activity was achieved under these conditions. The maximum yield of enzymes (62-72 U ml- ' of pectinase, 4-74 U ml- ' of polygalacturonase, 113.30 U ml-' of pectin lyase, 2-10 U ml-' of pectinesterase, 0.75 U ml- ' of total cellulase and 9.27 U ml-' of xylanase) was recorded with the consortia C-S2 developed from decomposed plant samples collected from a pond.

Item Type: Article
Uncontrolled Keywords: anaerobic degradation, enrichment, fermentation, pectinase activity
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 30 May 2016 07:32
Last Modified: 30 May 2016 07:32
URI: http://ir.cftri.com/id/eprint/3014

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