An esterification method for determining lipase activity.
Kiran, K. R. and Hari Krishna, S. and Suresh Babu, C. V. and Karanth, N. G. and Divakar, S. (2000) An esterification method for determining lipase activity. Biotechnology Letters, 22 (19). pp. 1511-1514.
Restricted to Registered users only
The choice of a lipase for an esterification reaction can be determined from the esterification reaction between butyric acid (0.16 M) and butanol (0.33 M) at 50 �C and agitated with 10 mg lipase. The decrease in butyric acid is measured by titration and 1 unit of lipase activity is defined as 1 �mol butyric acid consumed per min per mg lipase.
|Divisions:||Fermentation Technology and Bioengineering|
|Uncontrolled Keywords:||butyl butyrate, butyric acid, esterification, lipase|
|Subjects:||600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes|
|Depositing User:||Food Sci. & Technol. Information Services|
|Date Deposited:||06 Jun 2011 05:30|
|Last Modified:||28 Dec 2011 09:42|
Actions (login required)