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Partial specific volumes and interactions with solvent components of alpha-globulin from Sesmum indicum L. in urea and guanidine hydrochloride.

Prakash, V. (1982) Partial specific volumes and interactions with solvent components of alpha-globulin from Sesmum indicum L. in urea and guanidine hydrochloride. Journal of Biosciences, 4 (3). pp. 347-359.

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Abstract

The interaction of α-globulin with urea/guanidine hydrochloride was investigated by determining the apparent partial specific volumes of the protein in these solvents. The apparent partial specific volumes were determined both under isomolal and isopotential conditions. The preferential interaction parameter with solvent components calculated were 0.08 and 0.1 g of urea and guanidine hydrochloride respectively per g protein. In both the cases the interaction was not preferential with water. The total binding of denaturant to α-globulin was calculated both for urea and guanidine hydrochloride and the correlation between experimentally determined number of mol of denaturant bound per mol of protein and the total number of peptide bonds and aromatic amino acids were found to be in excellent agreement with each other. The changes in volume upon transferring α-globulin from a salt solution to 8 Μ urea and 6 Μ guanidine hydrochloride were also calculated.

Item Type: Article
Uncontrolled Keywords: Partial specific volume; denaturation; preferential interaction; α-globulin; urea and guanidine hydrochloride
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
600 Technology > 08 Food technology > 19 Lipids-oils/fats > 01 Oilseeds
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Mar 2018 07:03
Last Modified: 16 Mar 2018 07:03
URI: http://ir.cftri.com/id/eprint/2836

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