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Alpha-D-Mannosidase from Lycopersicon esculentum II.

Suvarnalatha, G. and Prabha, T. N. (1999) Alpha-D-Mannosidase from Lycopersicon esculentum II. Phytochemistry, 50 (7). 1111-1115, 19 ref..

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were puri_ed by ion!exchange chromatography on DEAE sephadex A!49 followed by gel _ltration on sephadex G!099[ The two isoforms were named isoform I and II "minor and major forms\ respectively# which di}ered in their abundance\ their elution pro_le on both ion!exchange and gel _ltration column chromatography[ Isoforms I and II showed 5 and 13) of the total activity\ respectively[ Both the isoforms had an acid pH optimum "3[4# and were thermally stable at 54> for up to 04 min[ Isoform I showed a broad temp optimum between 44 and 54> whereas isoform II had an optimal activity at 54>[ The Km values of isoforms I and II for p!nitrophenyl!a!D!mannopyranoside were 0[00 and 0[94 mM\ respectively[ The activity of isoform I was inhibited by Hg1� "0 mM# whereas isoform II was inhibited by Cu1� "9[0 mM# and Hg1� "0 mM#[ Both the isoforms were free of other glycosidases[ Puri_ed isoform II had a SDS Mr of ca 38,000.

Item Type: Article
Uncontrolled Keywords: Lycopersicon esculentum Solanaceae Tomato Enzyme purification Isoforms alpha-d-Mannosidase
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
600 Technology > 08 Food technology > 23 Vegetables > 03 Tomato
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 14 Feb 2012 09:06
Last Modified: 14 Feb 2012 09:06
URI: http://ir.cftri.com/id/eprint/2772

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