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Method for the isolation of gossypin (11S protein) and congossypin (7S protein) from glanded cottonseed.

Mohan Reddy, I. and Narasinga Rao, M. S. (1988) Method for the isolation of gossypin (11S protein) and congossypin (7S protein) from glanded cottonseed. Journal of Agricultural and Food Chemistry, 36 (2). pp. 237-240.

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Abstract

A method for the isolation of gossypin and congossypin from glanded cottonseed has been developed on the basis of their solubility characteristics as a function of ionic strength and temperature. Further purification of the proteins by gel filtration on Sephadex G-200 column yielded homogeneous (-98%) preparations, as judged by sedimentation velocity, gel electrophoresis, and ion-exchange chromatography. Gossypin and congossypin were characterized by sedimentation constants (S20,wof) 11.0 and 7.6 and E;& at 280 nm of 7.6 and 6.0, respectively. The proteins were free from gossypol (free and bound) and nucleic acid impurities.

Item Type: Article
Uncontrolled Keywords: isolation, gossypin, congossypin, glanded cottonseed
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
600 Technology > 08 Food technology > 19 Lipids-oils/fats > 03 Cotton seed
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Mar 2018 09:38
Last Modified: 16 Mar 2018 09:38
URI: http://ir.cftri.com/id/eprint/2711

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