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Aspartokinase of a lysine producing mutant of Micrococcus glutamicus

Meena, Lakshman. and Shenoy, B. C. and Raghavendra Rao, M. R. (1981) Aspartokinase of a lysine producing mutant of Micrococcus glutamicus. Journal of Biosciences, 3. pp. 57-67. ISSN 0021-8561

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Aspartokinase from Micrococcus glutamicus AEC RN-13-6/1 [a homoserine requiring, S-(2–aminoethyl)-L-cysteine resistant, lysine producing strain] was purified 71 fold. The partially purified enzyme was inhibited by L-lysine. L-threonine, L-methionine, Lisoleucine, L-valine and L-phenylalanine activated the enzyme and reversed the inhibition by Llysine. Aspartokinase activity was not derepressed by growth–limiting concentrations of Lthreonine and/or L–methionine. It was not repressed by an excess of L-lysine (20 mM) and/or L-isoleucine (15.3 mM). The degree of activation or inhibition by amino acids was dependant on the composition of the growth medium. This observation is in contrast with the enzyme from the original (non–lysine–producing) strain which was inhibited by lysine or threonine and in a concerted manner by threonine plus lysine.

Item Type: Article
Uncontrolled Keywords: Aspartokinase; Micrococcus glutamicus
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 19 Mar 2018 05:46
Last Modified: 19 Mar 2018 05:46
URI: http://ir.cftri.com/id/eprint/2650

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